Table 1 Primer sequences used in the real-time quantitative PCR analysis
Target gene | Forward primer | Reverse primer |
|---|---|---|
EZH2 | GAATGGAAGCAGCGAAGGATACAG | CAAGTCACTGGTCACTGAACACTCC |
p16 | CGTCGTGCGGTATTTGCGGTATC | CGTTGCCAGAAGTGAAGCCAAGG |
NOX4 | ATGGTGGTGGTATTGTTCCTCAT | CAGCAGCAGCAGCATGTAGA |
GAPDH | CGGCAAGTTCAACGGCACAGT | CGACATACTCAGCACCAGCATCAC |
β-Actin | TCAGGTCATCACTATCGGCAAT | AAAGAAAGGGTGTAAAACGCA |
β-Catenin | CATTACAACTCTCCACAACC | CAGATAGCACCTTCAGCA |
Wnt11 | ATGGCATCAAGTGGCTGGCATT | ATGGCATCAAGTGGCTGGCATT |
Wnt6 | ATGCTGCTGCTGCTGCTCTTGC | AACGGAACTGGAACTGACATTCTCG |
Mapk8 | ACAGTGAGCAGAGCAGGCATAGT | TTGTCAGGAGCAGCACCATTCTTAC |
Wif1 | CCAGGCGAGAACTTCACAAGCA | TAGCAGGAGCAGGCAAGGTAGG |