Fig. 3
Metaphase-blocked MCF-7 breast cancer cells form less cohesive clusters. a Aggregate circularity was determined in control (untreated) and metaphase-synchronized/blocked MCF-7 cells after 5 h. ***P < 0.001 (Mann–Whitney non-parametric test). b Cluster cohesion analysis using a flush-assay. After 5 h, clusters were mechanically dissociated by repeatedly flushing with a micropipette. The number of dissociated cells was determined. Data are the mean ± SD of 12 wells for each condition from 4 independent experiments. *P < 0.05; ***P < 0.001 (two-way ANOVA)