Fig. 1

Subcellular localization of RalA/B during mouse oocyte meiosis. A Results of the expression of RalA/B protein in different stages by Western blotting. B The band intensity analysis for the RalA/B. RalA/B were all expressed at GV, GVBD, MI and MII stages. C Subcellular RalA/B localization during mouse oocyte meiosis based on staining with an anti-RalA/B antibody. In GV stage, RalA/B were enriched inside the germinal vesicle. After GVBD, RalA/B were mainly around the chromosomes. When oocytes were in the MI and MII stages, RalA/B were mainly distributed around the spindle. Red, RalA/B. Bar = 20 µm. *P < 0.05, **P < 0.01