Fig. 3

Knockdown of RalA/B increases the actin density in mouse oocytes. A The typical picture of RalA/B and actin localization. RalA/B showed similar localization pattern with actin in the MI stage oocytes. DIC, differential interference contrast. Red, actin; Green, RalA/B; Bar = 20 μm. B The typical picture for the intensity of actin at cortex after RalA/B siRNAs injection. Red, actin. Blue, DNA. Bar = 20 µm. C The fluorescence intensity of actin at the cortex after RalA/B siRNAs injection. Compared with the control group, the relative fluorescence intensity of actin at cortex was significantly increased in RalA/B-KD group. *P < 0.05. D The typical picture for the intensity of actin in the cytoplasm after RalA/B siRNAs injection. Red, actin. Blue, DNA. Bar = 5 µm. E The fluorescence intensity of actin in the cytoplasm after RalA/B siRNAs injection. Compared with the control group, the relative fluorescence intensity of actin in the cytoplasm was significantly increased in RalA/B-KD group. *P < 0.05