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Table 2 lists the strains used for this study

From: The budding yeast protein Chl1p is required for delaying progression through G1/S phase after DNA damage

Strain

Genotype

Reference

699

MATa ade2-1 trp1-1 leu2-3, 112 his 3–11, 15 ura3 can1-100

[7]

699Dchl1

MATa ade2-1 trp1-1 leu2-3, 112 his 3–11, 15 ura3 can1-100 chl1::HIS3

[7]

US456

MATα leu2 his3 trp1 ade2 rad24::URA3

Uttam Surana

SL1

MATα leu2 his3 trp1 ade2 rad24::URA3 chl1::HIS3

This study, by crossing

US456 with 699Dchl1

SL3

MATa leu2 his3 trp1 ade2 rad24::URA3

By crossing US456 with 699Dchl1

SL4

MATa leu2 his3 trp1 ade2 rad24::URA3

chl1::HIS3

By crossing US456 with 699Dchl1

US355

MATα cdc13 rad9 leu2 ura3

Uttam Surana

SL9

MATa leu2 his3 trp1 ade2 ura3 rad9

By crossing US355 with 699

SL9DChl1

MATa leu2 his3 trp1 ade2 ura3 rad9 chl1::HIS3

This study, by disrupting CHL1 in SL9

US354

MATα leu2 his3 trp1 ade2 ura3 rad53-21

[7]

SL7

MATa leu2 his3 trp1 ade2 ura3 rad53-21

[7]

SL7Dchl1

MATa leu2 his3 trp1 ade2 ura3 rad53-21 chl1D::TRP1

This study, by deleting CHL1 in SL7

699Δsgs1

MATa ade2-1 trp1-1 leu2-3, 112 his 3-11,15 ura3 can1-100 sgs1Δ::LEU2

This study, by deleting

SGS1 in 699

699Δsgs1 Dchl1

MATa ade2-1 trp1-1 leu2-3, 112 his 311,15 ura3 can1-100 sgs1Δ::LEU2 chl1::HIS3

This study, by disrupting

CHL1 in 699Δsgs1

SL21

MATa ade2-1 trp1-1 his 3-11,15 ura3 can1-100 sgs1Δ::LEU2 rad24::URA3

This study, by crossing

SL1with 699Δsgs1

SL 26

MATa ade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 Chk1::LEU2

This study, by deleting

CHK1 in 699

SL27

MATa ade2-1 trp1-1 leu2-3, 112 his 311, 15 ura3 can1-100 chl1::HIS3 Chk1::LEU2

This study, by deleting

CHK1 in 699Dchl1

  1. 699 and all the strains listed are in W303 background
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Cell Division

ISSN: 1747-1028

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