Fig. 1

HPV16-E7 increases MPS1 transcript and protein levels. A MPS1 expression. MPS1 mRNA in U2OS-E7 cells (2 µg) was determined by qRT-PCR and analyzed in three independent experiments, a statistically significant increase (p < 0.0001) with respect to cells transfected with the empty vector (control) was observed. B Western Blot of MPS1. Protein levels were determined in U2OS control cells and U2OS cells transfected with increasing HPV16-E7 plasmid concentrations (2–8 µg, left panel). An increase in MPS1 protein levels was observed in U2OS-E7 (8 µg) with respect to control cells (right panel). Actin was used as a loading control. C Stabilization of MPS1 mediated by HPV16-E7. The half-life of MPS1 was determined by treating U2OS-E7 and control cells with cycloheximide at a final concentration of 100 µg/ml. MPS1 protein was more stable in U2OS-E7 cells than control cells