Fig. 2

HPV16-E7 increases the centrosome number in U2OS-E7 cells. A Immunodetection of centrosomes in cells transfected with HPV16-E7. Immunofluorescence was performed to detect γ-tubulin (red) and centrin (green), contrasting the nucleus with DAPI (blue). We found cells with a γ-tubulin cloud surrounding multiple centrin signals (II) or multiple centrosomes with γ-tubulin signals and two centrin signals (III). The objective used was 100X. B Percentage of cells with multiple centrosomes. Mononuclear cells with γ-tubulin and centrin signals were counted in three individual experiments. A statistically significant increase (11.3%, p < 0.01) was observed in cells transfected with HPV16-E7 compared to control cells