Skip to main content
Fig. 2 | Cell Division

Fig. 2

From: Heat shock factor 1 suppression induces spindle abnormalities and sensitizes cells to antimitotic drugs

Fig. 2

Inhibition of HSF1 transactivation activity during mitosis induces spindle abnormalities and alters the reassembly of mitotic spindles. a Treatment of KRIBB11 and CCT251263 at mitotic stage induced defects in mitotic spindles. CGL2 cells were treated with indicated concentrations of KRIBB11 or CCT251263 for 1 h and immediately subjected to analysis of mitotic spindles by immunofluorescence staining. The results (mean ± SD) were derived from at least 500 mitotic cells from three independent experiments. *p < 0.01 by Student’s t-test as compared with the untreated control. After treatment, the mitotic cells were also shaken off the plates and collected for immunoblot analysis of HSP70 (right panal). b Representative images of the mitotic spindle after nocodazole treatment (3 μM for 0.5 h) and washout for 0 (i), 3 (ii), and 30 min (iii). c CGL2 cells were treated with 40 μM KRIBB11 for 1 h; during the last 0.5 h of KRIBB11 treatment, nocodazole was added into culture medium at 3 μM. Nocodazole was then completely washed out, and the cells were incubated in drug-free medium for 3 min before being immediately fixed and immunostained for α-tubulin (red) and γ-tubulin (green). The chromosomes were counterstained with DAPI (blue). d The numbers of microtubule asters in cells. Results are the median ± 25th percentiles of at least 250 mitotic cells for each condition, as determined from three experiments. *p < 0.05 by Dunnett test as compare every median with a KRIBB11-untreated control median using the GraphPad Prism 9.1

Back to article page