Fig. 2

Localised activation of Plk1^Plo1 at the yeast SPB reinforces the pro-mitotic signalling to drive cells into mitosis. The recruitment of PP1^Dis2 to Cut12 negatively regulates the recruitment of Plk1^Plo1 at the yeast spindle pole body (SPB). In G2, 30 min before mitosis, Nek2^Fin1 and Cdk1^Cdc2 activation at the centrosome expels PP1^Dis2 from the SPB by phosphorylating the residues T75 T78 on Cut12. The disassociation of PP1^Dis2 from the SPB allows the recruitment and activation of Plk1^Plo1 at the SPB. The activation of Plk1^Plo1 promote the inhibition of Wee1 and facilitates Cdc25 activation, reinforcing the commitment into mitosis by favouring the activation of Cdk1^Cdc2