Fig. 3

dGLYAT is necessary for Eiger-JNK pathway-induced invasive cell migration. Fluorescent micrographs of third instar larval wing discs are shown (a-e). Compared with the ptc > GFP control (a), ptc > Egr-induced cell migration and up-regulated MMP1 expression (b) were suppressed by dGLYAT mutation (c) or RNAi (e), but not by LacZ expression (d). f Statistic of number of migrated cells is shown (left to right: n = 10, n = 12, n = 10. n = 12, n = 16). g Statistic of number of MMP1 positive dots is shown (left to right: n = 10, n = 22, n = 26. n = 27, n = 25). t-test was used to compute P-values. **P < 0.01, ****P < 0.0001. Detailed genotypes: a ptc-Gal4 UAS-GFP/+, b ptc-Gal4 UAS-GFP UAS-Egr ^Regg1/+, c ptc-Gal4 UAS-GFP UAS-Egr^Regg1/dGLYAT^c02982, d ptc-Gal4 UAS-GFP UAS-Egr^Regg1/+; UAS-LacZ/+, e ptc-Gal4 UAS-GFP UAS-Egr^Regg1/+; UAS-dGLYAT-IR/+