Fig. 4

dGLYAT is required for cell polarity disruption-induced JNK pathway activation. Light micrographs of third instar wing discs with X-gal staining (a-c) and fluorescent micrographs of third instar wing discs (d-f) are shown. Compared with the ptc-Gal4 control (a, d), ptc > scrib-IR activated puc-LacZ (b) and TRE-RFP (e) expression, which were suppressed by dGLYAT RNAi (c, f). Detailed genotypes: a ptc-Gal4 UAS-GFP/+; puc-LacZ/+, b ptc-Gal4 UAS-GFP UAS-scrib-IR/+; puc-LacZ/+, c ptc-Gal4 UAS-GFP UAS-scrib-IR/+; puc-LacZ/UAS-dGLYAT-IR, d ptc-Gal4 UAS-GFP/TRE-RFP, e ptc-Gal4 UAS-GFP UAS-scrib-IR/TRE-RFP, f ptc-Gal4 UAS-GFP UAS-scrib-IR/TRE-RFP; UAS-dGLYAT-IR/+