Fig. 6

Gadd45 knockdown suppresses scrib depletion-induced JNK activation and cell invasion. Compared with the ptc-Gal4 control (a, e, i), scrib RNAi-induced cell migration (b), elevated expression of MMP1 (f) and puc-LacZ (j) were significantly suppressed by expressing two independent Gadd45 RNAi (c, d, g, h and k, l). m Statistic of number of migrated cells is shown (left to right: n = 10, n = 12, n = 9. n = 10). n Statistic of number of MMP1 positive dots is shown (left to right: n = 9, n = 9, n = 9, n = 9). One-way ANOVA was used to compute P-values, ****P < 0.0001. Larvae were reared at 25 °C. Detailed genotypes: a, e, i ptc-Gal4 UAS-GFP/+; puc-LacZ/+, b, f ptc-Gal4 UAS-GFP UAS-scrib-IR; puc-LacZ /UAS-LacZ, c, g, k ptc-Gal4 UAS-GFP UAS-scrib-IR/UAS-Gadd45-IR^V100413; puc-LacZ/+, d, h, l ptc-Gal4 UAS-GFP UAS-scrib-IR/UAS-Gadd45-IR^BL35023; puc-LacZ/+, j ptc > GFP UAS-scrib-IR; puc-LacZ/UAS-GFP