Fig. 1

Proliferation of PTIP RNAi cells. A HeLa Tet-on cells were arrested by thymidine (G1), RO3306 (G2), or nocodoazole (M), and analyzed for PTIP protein level. B HeLa Tet-on cells were transfected with three different PTIP siRNA oligos for 36 h and DIC images were taken. White arrow indicates an example of blebbing cells. C Cells from B were counted by bright spot detection function of Nikon Eclipse software after staining DNA with Hoechst 33,342. Three replicates of treatments were used for analysis. P-value was calculated by student t-test, paired, two-tailed. D HeLa Tet-on cells were transfected with three different PTIP siRNA oligos for 36 h and total lysates of cells were resolved in SDS-PAGE and processed for western blot using PTIP antibody and PARP antibody. Arrows indicate full-length PTIP or PARP proteins. Circle indicates cleaved PARP and square indicates non-specific protein.