Fig. 2

MiR-146b-5p promotes migration, invasion and EMT of ccRCC cells. A, B: Transwell assay and wound healing experiment were employed to determine the migratory and invasive abilities of ccRCC cells 786–O and Caki-2 in different treatment groups (100 ×); C: Western blot assay was employed to measure the expression levels of MMP2 and MMP9 in 786–O and Caki-2cells in different treatment groups; D: The morphology of 786–O and Caki-2 cells in each transfection group was observed under microscope; E: qRT-PCR evaluated the mRNA levels of Notch1, Snail1, Snail2, E-cadherin, N-cadherin and Vimentin in 786–O and Caki-2 cells; F: The protein expression of Notch1, E-cadherin, N-cadherin, Vimentin, Snail1, Snail2, and GAPDH in ccRCC cells 786–O and Caki-2 in different treatment groups; All the above experiments were performed with 3 biological replicates; * P < 0.05 indicates statistical difference, **P<0.01 indicates significant difference, ***P<0.001 indicates extremely siginficant difference.