Fig. 3

MiR-146b-5p targets the expression of SEMA3G A: Venn diagram of miR-146b-5p predicted target mRNA and down-regulated differential mRNAs. Green indicates TargetScan database, orange indicates down-regulated differential mRNAs in TCGA-KIRC dataset, yellow indicates starBase database, pink indicates mirDIP database, blue indicates miRDB database; B: Pearson correlation analysis plot of miR-146b-5p and candidate genes; C: Pearson correlation analysis plot of miR-146b-5p and SEMA3G; D: Expression of SEMA3G in TCGA-KIRC database, blue box plot indicates normal samples, yellow box plot indicates tumor samples; E: Expression level of SEMA3G on overall survival curve of patients. Red indicates high expression of SEMA3G. Blue represents low SEMA3G expression; F: SEMA3G mRNA expression level in normal cell line HK-2 and ccRCC cell lines 786–O, A498, 769-P and Caki-2; G: Schematic diagram of SEMA3G-WT and SEMA3G-MUT binding to miR-146b-5p sequence predicted by starBase; H: Dual-luciferase assay was used to detect luciferase activity in different treatment groups in ccRCC cell line Caki-2; I: qRT-PCR was performed to detect the regulatory relationship between miR-146b-5p and SEMA3G in Caki-2 cells; All the above experiments were performed with 3 biological replicates; *P < 0.05 indicates statistical difference, **P<0.01 indicates significant difference, ***P<0.001 indicates extremely significant difference.