Fig. 4

MiR-146b-5p targets SEMA3G to regulate Notch and TGF-β signaling pathways to affect the migration, invasion and EMT of ccRCC cells. A: GSEA pathway enrichment analysis results of SEMA3G; B, C: The mRNA and protein expression of SEMA3G in different treatment groups of ccRCC cells Caki-2; D, E: Transwell assays and wound healing experiment were performed to inspect the migratory and invasive abilities of ccRCC cells Caki-2 in different treatment groups (100 ×); F: Western blot was performed to inspect the expression levels of MMP2 and MMP9 in Caki-2 cells; G: The morphology of Caki-2 cells in each transfection group was observed under microscope; H: qRT-PCR evaluated the mRNA levels of Notch1, Snail1, Snail2, E-cadherin, N-cadherin and Vimentin in Caki-2 cells; I: The expression of EMT marker proteins, Notch, and TGF-β signaling pathway marker proteins in different treatment groups of ccRCC cells Caki-2; All the above experiments were performed with 3 biological replicates; *P < 0.05 indicates statistical difference, **P<0.01 indicates significant difference, ***P<0.001 indicates extremely significant difference. One-way ANOVA analysis of variance was used for comparison of three groups.