Figure 1
From: Modification of Cul1 regulates its association with proteasomal subunits
Co-immunoprecipitation of SCF subunits with proteasome components. (A) Extracts from Hela cells were immunoprecipitated with a polyclonal antibody that recognizes multiple proteasomal subunits (lane 1) or a nonrelevant polyclonal serum (lanes 2). Lane 3 contains whole cell lysate. Immunoprecipitates were analyzed by immunoblotting with antibodies to the indicated proteins. A non-specific cross-reactive band is indicated by the asterisk. (B) Extracts from Hela cells were immunoprecipitated with a monoclonal antibody to alpha 6 (lane 1), a monoclonal antibody to alpha 2 (lane 4), a monoclonal antibody to alpha 4 (lane 5), a nonrelevant supernatant (lane 2) or mouse IgG (lane 6). Lanes 3 and 7 contain whole cell lysate. Immunoprecipitates were analyzed by immunoblotting with antibodies to the indicated proteins.