Figure 6
From: Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast
Tup1 occludes Gcn5 recruitment. A) ChIP was performed using the cells shown expressing GAL pro -GCN5-HA following a 5 hour galactose induction, as described above. (B) Two independent experiments were scanned and processed using ImageJ, with the means and standard errors shown. (C) Strains lacking TUP1 were constructed in WT and apc5CA backgrounds. Growth phenotypes were assessed by spot-dilutions, followed by incubation at 34°C and 37°C.