Figure 4

Nuclei morphological changes during PTO and DDPT-induced apoptosis in tumor cells detected by AO/EB staining. After treated with PTO and DDPT at 20 μ M, PC3 and Bcap-37 cells were stained with AO/EB (100 µ g/mL) and observed under fluorescence microscopy. For PC3 cells group, A: negative control (without treatment); D: positive control, treated with HCPT (20 µ M) for 24 h; B and C: treated with PTO (20 μ M) for 24, 48 h; E and F, treated with DDPT (20 µ M) for 24, 48 h, respectively. For Bcap-37 cells group, A': negative control; D': positive control, treated by HCPT (20 μ M) for 24 h; B' and C': treated with PTO (20 μ M) for 24, 48 h; E'and F': treated with DDPT (20 μ M) for 24, 48 h, respectively.