Table 2 Plasmids used in this study.

pYL150

2μm, LEU2, Amp ^r , GAL10 promoter +CDC34

[17]

pYL19

pYL150 with S97D mutation in CDC34

[17]

pYL27

pYL150 with 12-residue deletion of G-103 to T-114 in CDC34

[17]

pYL29

pYL150 with 12-residue deletion of G-103 to T-114 and S73K and S97D mutations in CDC34

[17]

pUBP14

ADH1 promoter and terminator elements + UBP14 in pVT102-U

[38]

pUBP14-Ala354

ADH1 promoter and terminator elements +catalytically inactive UBP14(C354A) mutant in pVT102-U

[38]