Figure 3From: The yeast mitogen-activated protein kinase Slt2 is involved in the cellular response to genotoxic stress Analysis of the hypersensitivity of slt2 cells to genotoxic stress and the activation of Slt2 by genotoxic stress in osmotically supported medium. A) 10-fold serial dilutions from exponentially growing cultures of wild type (W303-1a) and slt2 (JCY1062) strains were spotted onto YPD medium containing 1 M sorbitol and 200 mM hydroxyurea or 0.03% MMS or were exposed to UV radiation (40 J/m2). Plates were incubated at 25°C for 3 days. B) Cultures of the wild type W303-1a strain grown on YPD containing 1 M sorbitol were split and incubated for 60 min in the absence or presence of 200 mM hydroxyurea, 0.04% MMS or 5 μg/mL phleomycin, or were irradiated with UV radiation (50 J/m2). The level of phosphorylated Slt2 and the chekpoint kinase Rad53 (as a control of the presence of genotoxic stress) was determined by western analysis. The ponceau staining of the membrane is shown as loading control.Back to article page