| Daughter | Mother | Overall |
---|
Strain | Birth size | B1 size | G1 (min) | B2 size | CT | B1 size | CT1 | B2 size | CT2 | B3 size | G1 (min) | CT | RGR |
---|
WT | 5.4 | 5.8 | 32 | 6.0 | 83 | 6.3 | 75 | 6.4 | 74 | 6.4 | 4 | 77 | 100% |
mrpl49Δ
| 4.2 | 5.1 | 172 | 5.2 | 167 | 5.5 | 140 | 5.6 | 154 | 5.7 | 30 | 154 | 51% |
rpl42aΔ
| 4.4 | 5.1 | 94 | 5.4 | 131 | 5.5 | 97 | 5.5 | 115 | 5.6 | 8 | 114 | 58% |
yjr114wΔ
| 4.5 | 5.4 | 136 | 5.5 | 183 | 5.8 | 136 | 5.9 | 137 | 6.0 | 25 | 152 | 67% |
rpl36bΔ
| 4.6 | 5.4 | 131 | 5.6 | 158 | 5.7 | 143 | 5.8 | 139 | 5.8 | 24 | 147 | 67% |
rom2Δ
| 4.6 | 5.1 | 89 | 5.3 | 135 | 5.6 | 115 | 5.6 | 111 | 5.7 | 4 | 120 | 64% |
cbs1Δ
| 4.6 | 5.6 | 141 | 5.9 | 208 | 6.0 | 129 | 6.1 | 150 | 6.2 | 26 | 162 | 69% |
rpl16bΔ
| 5.0 | 5.5 | 47 | 5.8 | 124 | 5.9 | 94 | 6.0 | 100 | 6.1 | 6 | 106 | 83% |
ctr9Δ
| 5.5 | 6.5 | 130 | 6.8 | 153 | 7.1 | 110 | 7.2 | 145 | 7.3 | 20 | 136 | 116% |
ecm9Δ
| 6.1 | 6.6 | 41 | 7.0 | 127 | 7.0 | 114 | 7.2 | 107 | 7.3 | 8 | 116 | 155% |
- Data gathered from microscopic analyses of >30 individual cells over a 6–10 hour time course is presented. Cell diameters are in microns. In each case, the birth diameter of daughter cells is shown. In addition, the size at which cells bud for the first time (B1) and subsequent buds (B2, etc.) are also provided. The length of G1-phase is directly measured as the time cells remain unbudded. Cycle time (CT) is measured from the time a cell first buds until its subsequent bud. Overall cycle time (CT) is shown as the average of one daughter CT and the first two mother cell cycle times. The relative growth rate (RGR) is a measure of the total cell volume produced per generation and is determined by calculating the change in volume of mother cells in each generation added, to the volume of daughter cells produced. For volume calculations, cells are assumed to resemble spheres and the formula 4/3πr3 is used. Wild type (WT) cells are set to 100% and all mutants are subsequently normalized to this value.