Figure 5
From: APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage
The SAC is still active during mitotic slippage. (A, B) Cells of strains SCU1337 (MAD2-GFP) and SCU1338 (bub2Δ MAD2-GFP) harboring the plasmid pSCU1701 (pMTW1-RFP) were released from α-factor into the nocodazole-containing medium (time 0), as described in Figure 1. Kinetochore localization of GFP-tagged Mad2 was monitored and counted after 6 h. White arrows indicate colocalized Mad2-GFP and Mtw1-RFP signals.