Table 1 Primers sequences used for real-time PCR and siRNA duplexes

Genes

Primer sequences

AURAK_F

5′-ATA TCT CAg Tgg Cgg ACg Ag-3′

AURAK_R

5′-TCA AAT ATC CCC gCA CTC Tgg-3′

CDC14B_F

5′-CTC CAT gAA gCg gAA AAg Cg-3′

CDC14B_F

5′-gCA AAA CAA Agg CgA TCg gT-3′

CDK1_F

5′-AAA CTg gCT gAT TTT ggC CT-3′

CDK1_R

5′-ggA gTg CCC AAA gCT CTg AA-3′

CEP192_F

5′-CCC gAg CAC TTg ATT CTg gT-3′

CEP192_R

5′-CCA CTC CAC ggg AAC ATT gA-3′

CETN2_F

5′-AgC ggA CTC CTT Tgg CTA Tg-3′

CETN2_R

5′-gCT CAg gCT TAg ggC TCA TT-3′

GINS2_F

5′-gCC gAg AAg gAg CTg gTT AC-3′

GINS2_R

5′-AAC CAg ggT TAA AAg gCC CC-3′

ROCK2_F

5′-CCC ATC AAC gTg gAg AgC TT-3′

ROCK2_R

5′-TgC CTT gTg ACg AAC CAA CT-3′

SASS6-F

5′-TAC ggA ATg AAT ggg CgT CA-3′

SASS6_R

5′-CTg TgC CTg CAA ggC TTT TT-3′

SPICE_F

5′-ggT CCC CgA gTT ggT gTA Ag-3′

SPICE_R

5′-gCg TAC CAg ATC TTC ggg Ag-3′

SGOL1_F

5′-Agg CAA AAg ATg gCC AAg gA-3′

SGOL1_R

5′-AAA gAC CTg CgT TTg CCA AT-3′

CDK14 F

5′-AAT gAg gAC ACA Tgg CCT gg-3′

CDK14_R

5′-CTg TgC CgA CAg TCT gTT CT-3′

c-Nap_F

5′-AAC CAg CTC Cgg gAg AAA Tg-3′

c-Nap_R

5′-TCT ggC ATA ggg CAC TCT CT-3′

MDM2_F

5′-CCA TgC CTg CCC ACT TTA gA-3′

MDM2_R

5′-CAg gCT gCC ATg TgA CCT AA-3′

PlexinA2_F

5′-ATT TTT CAg CCg AgA ggg Cg-3′

PlexinA2_R

5′-TTT TTC CAg CgC gAC TTT CC-3′

SEMA6A_F

5′-TgA TgC CAA ACA TgC CAA Cg-3′

SEMA6A_R

5′-gCg TCA ATg gCA Agg AAg TC-3′

SFRP1_F

5′-CTC AAC AAg AAC TgC CAC gC-3′

SFRP1_R

5′-CTC gTT gTC ACA ggg Agg AC-3′

Actin_F

5′-CgA ggC CCA gAg CAA gAg-3′

Actin_R

5′-CgT CCC AgT Tgg TAA CAA TgC-3′

TTK_F

5′-CgC AgC TTT CTg TAg AAA TggA-3′

TTK_R

5′-gAg CAT CACTTAGCGGAACAC-3′

siRNA duplexes

MDM2_1 Sense

5′-rGrGrA rCrCrU rUrGrU rArCrA rArGrA rGrCrU rUrCrA rGrGA A-3′

MDM2_1 Anti-Sense

5′-rUrUrC rCrUrG rArArG rCrUrC rUrUrG rUrArC rArArG rGrUrC rCrUrU-3′

MDM2_2 Sense

5′-rCrCrA rArGrA rCrArA rArGrA rArGrA rGrArG rUrGrU rGrGA A-3′

MDM2_2 Anti-Sense

5′-rUrUrC rCrArC rArCrU rCrUrC rUrUrC rUrUrU rGrUrC rUrUrG rGrGrU-3′

SFRP1_2 Sense

5′-rGrArA rGrCrA rArCrA rGrCrU rUrCrA rGrArA rArGrA rGrCT C-3′

SFRP1_2 Anti-Sense

5′-rGrArG rCrUrC rUrUrU rCrUrG rArArG rCrUrG rUrUrG rCrUrU rCrCrU-3′

SFRP1_3 Sense

5′-rGrArA rArCrA rUrUrU rCrCrU rUrUrG rArArC rUrUrG rArUT G-3′

SFRP1_3 Anti-Sense

5′-rCrArA rUrCrA rArGrU rUrCrA rArArG rGrArA rArUrG rUrUrU rCrUrU-3′

TTK_1 Sense

5′-rGrGrArGrGrUrUrCrArArGrCrArArGrGrUrArUrUrUrCrAGG-3′

TTK_1 Anti-Sense

5′rCrCrUrGrArArArUrArCrCrUrUrGrCrUrUrGrArArCrCrUrCrCrArC-3′

TTK_2 Sense

5′-rCrCrArGrArArUrCrCrUrGrCrUrGrCrArUrCrUrUrCrArAAT-3′

TTK_2 Anti-Sense

5′-rArUrUrUrGrArArGrArUrGrCrArGrCrArGrGrArUrUrCrUrGrGrUrU-3′

SGOL1_1 Sense

5′-rGrArArArUrArUrGrUrUrCrCrUrCrUrGrGrArArUrGrGrACC-3′

SGOL1_1 Anti-Sense

5′-rGrGrUrCrCrArUrUrCrCrArGrArGrGrArArCrArUrArUrUrUrCrCrU-3′

SGOL1_2 Sense

5′-rGrGrArCrUrArCrArGrGrCrArUrGrUrGrCrCrArCrUrArCGC-3′

SGOL1_2 Anti-Sense

5′-rGrCrGrUrArGrUrGrGrCrArCrArUrGrCrCrUrGrUrArGrUrCrCrCrA-3′