Fig. 1

EZH2 inhibition induced apoptosis in NRK-52E cells and inhibited of HuR expression. NRK-52E cells were stimulated with 0, 10, 20, and 40 μM DZNep (3-deazaneplanocin A) for 24 h. Flow cytometry analysis revealed obvious apoptosis after DZNep treatment. Quantitative analysis of cell apoptosis was performed by flow cytometry, and apoptotic cells were identified as Annexin V⁺7-AAD⁻ (a). NRK-52E cells were treated with various concentrations of DZNep for 24 h and the percentage of apoptotic cells was measured (b). NRK-52E cells were stimulated with 20 μM DZNep for the indicated times (c, d) or stimulated with different concentrations of DZNep for 24 h (e, f). Western blots showed the protein level of EZH2, cleaved-caspase 3, HuR and Bcl-2; GAPDH was used to verify equivalent loading. Quantitative data are presented. Data are given as the mean ± SD of three independent experiments. *p < 0.05 versus vehicle-treated cells