Fig. 2

XMU-MP-1 antagonises cell cycle-dependent paclitaxel cytotoxicity. a, b XMU-MP-1 blocks the accumulation of phospho histone H3 (pH3)+ and cleaved caspase 3+ (CC3) cells in the hair matrix seen following paclitaxel treatment [2]. 50 µm scale. Analysis conducted using 4-7 hair follicles per condition from 2 donors. c Paclitaxel blocks nascent RNA synthesis, as visualised by 5-ethynyl uridine (EU) incorporation, in abnormally dividing hair matrix keratinocytes (white arrows) [2, 14]. This paclitaxel-induced block in RNA synthesis is prevented by XMU-MP-1 treatment. d, e Treatment with the Aurora B inhibitor AZD1152 also blocks the accumulation of pH3 + cells in the hair matrix following paclitaxel treatment, but results only in a trending reduction in the number of cleaved caspase 3 + cells, possibly due to the independent cytotoxicity of AZD1152 [16]. Analysis conducted using 4–5 hair follicles per condition from 1 donor. Adjusted p values = 0.0006 [***] and < 0.0001 [****]. Ordinary one-way ANOVA with multiple comparisons performed