Fig. 4From: ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigsKnockdown of ROMO1 induces apoptosis in porcine embryos. A TUNEL assay and apoptosis index in control and ROMO1 KD blastocysts. B Bcl-xL levels in control and ROMO1 KD blastocysts. C Colocalization of cytochrome c and MitoTracker Red in control and ROMO1 KD 4-cell stage embryos. Scale bars, 20 μm. D Western blots of OPA1, OMA1, and ROMO1 in control and ROMO1 KD groups. *P < 0.05 and **P < 0.01 indicate significant differences between treatment groupsBack to article page