Fig. 1

SIRT4 is downregulated in bladder cancer and is an independent prognostic factor for it. A SIRT4 is localized in the cytoplasm; SIRT4 staining is downregulated in tumor tissues compared to adjacent nontumorigenic bladder tissues. Paraneoplastic nontumor tissue is shown on the left, and tumor tissue is shown on the right. The lower images are magnified images in the box above. (Magnification: upper border 100× , lower border 400 ×). B Staining levels of SIRT4 in 56 BLCA tissues with 59 nontumor bladder tissues were analyzed by tissue microarray. SIRT4 protein levels were significantly reduced in tumor tissues compared with adjacent nontumorigenic bladder tissues (P < 0.01). Boxes represent interquartile range; whiskers represent 5th–95th percentile range; bars represent median. C The staining fraction of SIRT4 in different T-stages. It can be seen that the later the T-stage, the lower the staining score of SIRT4. D The staining fraction of SIRT4 in different AJCC stages. It can be seen that the later the AJCC stage, the lower the staining score of SIRT4. E Overall survival time of BLCA patients with low SIRT4 levels was significantly lower than that of patients with high SIRT4 levels by staining score analysis of immunohistochemistry (P < 0.01). F Cox multivariate analyses were performed on the overall survival time of BLCA patients. SIRT4 is the independent prognostic factor for BLCA patients