Fig. 2

SIRT4 inhibits the proliferation, migration and invasion of BLCA cells in vitro. A T24 cells transfected with oeSIRT4 or shSIRT4, and the effect of overexpression or interferencing of SIRT4 was detected by Western blotting, with GAPDH as an internal control (Uncropped gel in supply Fig. 1). B and C Proliferation viability of T24 cells after overexpression (B) or interference (C) with SIRT4 was assayed with CCK-8 reagent. For figure C, cell proliferation viability was measured on the fifth day after seeding. D and G Effect of overexpression (D) or interfering (G) with SIRT4 on the scratch healing ability of T24 cells. Detection was performed after 24 h of serum-free culture after scratching. E and H Effect of overexpression (E) or interference (H) with SIRT4 on the migration ability of T24 cells. Counting was performed after taking random images of 5 areas in each well with a 400 × microscope. F and I Effect of overexpression (F) or interference (I) with SIRT4 on the invasive ability of T24 cells. Images of 5 areas were taken randomly in each well with a 400 × microscope after counting. *P < 0.05, **P < 0.01