Fig. 3

Overexpression with SIRT4 inhibit cell cycle and promotes apoptosis in T24 cells. A The effect of overexpression of SIRT4 on the cell cycle distribution of T24 cells was analyzed by PI Cell Cycle Assay Kit. Modfit (Verity Software House, Topsham, ME, USA). B Flow cytometry (C6; BD, Franklin Lakes, NJ, USA) to detect the effect of overexpression SIRT4 on apoptosis of T24 cells. APC and 7-AAD were used as staining agents. The statistical graph on the right calculates the percentage of stained by APC, i.e., early and mid-late apoptosis. C Effect of interference with SIRT4 on apoptosis-related proteins caspase 3, caspase 9 and p65 in T24 cells was detected by Western Blot. Band density was analyzed and quantified using ImageJ software (version 2.1.4.7; National Institutes of Health, Bethesda, MD, USA). *P < 0.05, **P < 0.01 (Uncropped gel in supply Fig. 2)