Fig. 4

SIRT4 inhibits BLCA growth through autophagy inhibition. A RFP-GFP-tagged LC3 adenovirus was utilized to infect stably transfected T24 cell strains using SIRT4 overexpression and interference and negative control cells. Green dots indicate autophagosomes, yellow dots (marked in green and red) depict autophagic lysosomes without fusion with the lysosomes, and RFP signals without GFP relate to the fusion of autophagosomes with lysosomes. The yellow fluorescent particles within the cells were observed under the fluorescence microscope. B After 100 nmol/L bafilomycin treatment for 2 h, the changes in autophagy-related proteins LC3, SQSTM1, BECN1, GABARAP, and GABARAPL2 in T24 cells with SIRT4 overexpression and interference were detected (Uncropped gel in supply Fig. 3). C The inhibition effect on T24 cells with SIRT4 overexpression in the presence or absence of 100 nmol/L bafilomycins was detected using CCK-8. D Western blot analysis shows the interference effect of the shATG5 interference sequence on the ATG5 protein in T24 cells (Uncropped gel in supply Fig. 4). E In the presence or absence of shATG5, the inhibition effect on T24 cells with SIRT4 interference was detected by CCK-8