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Fig. 2 | Cell Division

Fig. 2

From: Rapid and accurate genotyping of human SNP rs671 in aldehyde dehydrogenase 2 gene using one-step CRISPR/Cas12b assay without DNA amplification

Fig. 2

Performance and sensitivity of CRISPR/Cas12b assay for three genotypes of ALDH2 rs671 SNP. The panels represent tenfold gradient concentrations of human gDNA: A 104 copies/rxn, B 103 copies/rxn, C 102 copies/rxn and D 101 copies/rxn. For each panel, left is fluorescence curves for 30 min continuous acquisitions, and the right bar graph represents the fluorescence values at the endpoint of 30 min. Error bars represent show the mean ± standard deviation from three replicates. Multiple comparisons between different groups were performed by one-way ANOVA. The groups exhibiting significant differences are annotated with distinct Latin letters, whereas groups with no statistically significant differences are labeled with the same Latin letter

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Cell Division

ISSN: 1747-1028

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