Fig. 1

Treatment with mTOR activator MHY1485 counteracted the effects of METTL3 on autophagy and proliferation of VSMCs. A and B The expression levels of METTL3, p-mTOR, and mTOR in HASMCs with or without METTL3 overexpression and MHY1485 (10 µM) were examined by using western blotting (A); Quantitative results (B) (n = 4). C-F The protein levels of autophagy markers ATG5, ATG7, and LC3 were detected by using western blotting in the HASMCs with indicated treatments (C); Quantitative results of blots in C (D–F) (n = 4). G–I The representative blots of proliferation markers p-H3 and PCNA were detected in HASMCs with indicated stimulations (G); Quantitative results (H and I) (n = 4). β-actin serves as loading control. *p < 0.05 versus lenti-Flag + DMSO. #p < 0.05 versus lenti-METTL3 + DMSO