Fig. 2

MHY1485 negatively regulated migration and synthetic phenotype of VSMCs, but cannot nullify the effects of METTL3 overexpression on these biological processes. A and B The HASMCs with lenti-METTL3 or lenti-Flag infection were treated with DMSO or MHY1485 (10 µM) for 24 h, and the migration of HASMCs was measured by using transwell assay (A); The quantitative results of migrated cells per high power field (B) (n = 10). Scale bar: 100 μm. C–E The protein levels of contractile markers α-SMA and SM22α were evaluated by western blotting (C); (D and E) The quantitative results of blots in C (n = 4). β-actin serves as loading control. *p < 0.05 versus lenti-Flag + DMSO