Fig. 3

Rapamycin offset the impacts of METTL3 knockdown on autophagy and proliferation. A–D The representative blots of METTL3, p-mTOR and mTOR in HASMCs with indicated treatments (rapamycin: 150 nM); B–D Quantification analysis of METTL3, p-mTOR and mTOR protein levels in A (n = 4). E–H The protein levels of ATG5, ATG7 and LC3 were detected by using western blotting in HASMCs with indicated stimulus (E); F–H Quantitative results of E (n = 4). I–K Western blotting was used to detect the protein levels of proliferation markers p-H3 and PCNA in HASMCs with indicated treatments (I); J and K Quantitative results of blots in I (n = 4). β-actin serves as loading control. *p < 0.05 versus lenti-shRNA + DMSO; #p < 0.05 versus lenti-shRNA + Rapamycin; &p < 0.05 versus lenti-shRNA + lenti-shMETTL3-2