Fig. 1

Slik inhibits ectopic Hep-induced cell death. Light micrographs of Drosophila adult eyes A–D and fluorescence micrographs of third instar larval eye discs F–I are shown. Compared with the GMR-Gal4 controls (A, F, GMR > Hep^CA-induced small eye phenotype B and cell death in eye discs G were partially inhibited by overexpression of Slik C, H, whereas enhanced in heterozygous slik mutants D, I. Statistical analysis of the adult eye sizes (E, n = 10 for each genotype) and AO-positive cell numbers in eye discs (J, n = 10 for each genotype) are shown. Light micrographs of Drosophila adult wings K–N and fluorescence micrographs of third instar larval wing discs P–S are shown. Compared with the ptc-Gal4 controls K, P, ectopic expression of Hep driven by ptc-Gal4 generated a loss-of-ACV phenotype in adults L and induced cell death in larval wing discs (Q), which were strongly blocked by expressing Slik (N, S), but not LacZ (M, R). The lower panels show high magnification view of the boxed areas in upper panels K–N). Quantification of the presence of ACV in adult wings (O, n = 15 for each genotype) and cell death in wing discs (T, n = 10 for each genotype) are shown. One-way ANOVA was used to compute P-values, ****p < 0.0001, ***p < 0.001, **p < 0.01, *P < 0.05, n.s indicates not significant. See the supplementary material for detailed genotypes. Scale bar:100 μm in A–D, F–I, K–N (upper panels) and P–S, 50 μm in K–N (lower panels)