Fig. 2

Depletion of slik triggers apoptotic cell death. Fluorescence micrographs of third instar larval eye discs A–D are shown. Compared with the GMR-Gal4 controls (A), downregulation of slik with three independent RNA interference (RNAi) triggered extensive cell death posterior to the MF (B–D). Fluorescence micrographs of third instar larval wing discs F–I, K–N are shown. Compared with the ptc-Gal4 controls (F, K), depletion of slik induced massive cell death (G–I) and apoptosis L–N in wing discs. Quantification of cell death in eye discs (E, n = 12; n = 9; n = 10; n = 9) and statistical analysis of AO positive cell number (J, n = 10 for each genotype) and cleaved Dcp-1 (cDcp-1) activity (O, n = 10 for each genotype) in wing discs are shown. P The knock-down efficacies of slik RNAi lines. Expression of three independent slik RNAi significantly reduces the level of slik mRNA, as measured by quantitative RT-PCR. One-way ANOVA with Bonferroni multiple-comparison test was used to compute P-values, ****P < 0.0001, ***P < 0.001. See the supplementary material for detailed genotypes. Scale bar: 50 μm