Fig. 6

Human STK10 reliefs slik depletion-caused cell death. Light micrographs of adult wings (A–D) and fluorescence micrographs of third instar larval wing discs F–I, K–N are shown. Compared with the controls (A, F), loss of slik driven by ptc-Gal4 resulted in reduced L3-L4 area in adult wings B and intense AO staining in wing discs (G), which were effectively rescued by expressing Slik (C, H) or STK10 (D, I). Statistical analysis of the adult wing L3-L4 size/total size ratio (E, n = 10 for each genotype) and cell death number (J, n = 10 for each genotype) in wing discs are shown. Comparable with the en-Gal4 control (K), slik knockdown-induced GFP area reduction in the posterior compartment L was restored by expressing Slik M or STK10 N. Statistical analysis of GFP⁺ area/GFP⁻ area ratio in K-N is shown (O, n = 10 for each genotype). One-way ANOVA with Bonferroni multiple-comparison test was used to compute P-values, ****P < 0.0001, n.s indicates not significant. See the supplementary material for detailed genotypes. Scale bar: 100 μm