Fig. 7

Human STK10 inhibits JNK signaling in Drosophila. Light micrographs of Drosophila adult eyes (A–C) and wings (I–K), and fluorescence micrographs of third instar larval eye (E–G) and wing discs (M–O) are shown. Compared with the controls (A, E), GMR > Hep^CA-evoked small eye phenotype (B) and cell death in eye discs (F) were inhibited by expressing STK10 (C, G). Statistical analysis of the adult eye sizes (D, n = 10 for each genotype) and AO-positive cell numbers in eye discs (H, n = 10 for each genotype) are shown. Consistently, compared with the ptc-Gal4 controls (I, M), ectopic Hep induced a loss-of-ACV phenotype in adult wings (J) and robust cell death in wing discs (N), both of which were suppressed by expressing STK10 (K, O). The lower panels show high magnification view of the boxed areas in upper panels (I–K). The presence of ACV in adult wings (L, n = 15 for each genotype) and AO positive cell numbers in wing discs (P, n = 10 for each genotype) were quantified. One-way ANOVA with Bonferroni multiple-comparison test was used to compute P-values, ****p < 0.0001, ***p < 0.001. See the supplementary material for detailed genotypes. Scale bar: 50 μm in A–C, E–G, I–K (lower panels) and M-O, 100 μm in I–K (upper panels)