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Fig. 1 | Cell Division

Fig. 1

From: Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity

Fig. 1

Typical microfluorimetric analyses of the DNA cellular content after Hoechst 33342 fluorochromization. In a PEG-untreated, in b PEG-treated NIHs cells, in c and d the measurements after one (c) and five (d) trypsinizations of PEG-treated cultures are reported, respectively. The smaller histogram (a1) inserted in a shows the microfluorimetric measurement of the NIH/3T3 cell line DNA content before serum starvation. During this analysis, a sample of mouse lymphocytes was measured in parallel to obtain the position of the value 2c in the abscissa axis (logarithmic scale). Although NIH/3T3 cells are hypertriploids (3–4c DNA content), NIHs cells derived from this line have been arbitrarily considered diploids to simplify the representation of data in various histograms. Therefore, in a, b, c, d, the first peak (3–4c DNA content) in the abscissa axis of microfluorimetric histogram was arbitrarily indicated as 2c. Overall histogram evaluations show the dynamics of the increase/decrease and/or appearance/disappearance of cells with different polyploid/aneuploid levels. Phase contrast micrographs inserted in a, b, c, and d, show the morphology of NIHs cultures under the respective experimental conditions

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Cell Division

ISSN: 1747-1028

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