Skip to main content
Fig. 7 | Cell Division

Fig. 7

From: Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity

Fig. 7

Degenerative aspects of cytoplasm and nuclear changes. Acridine orange (AO) fluorescence (a, b) and TEM (c, d) micrographs of PEG-treated polyploid NIHs cells. Images in b and d refer to cultures subsequently treated with vinblastine. It is possible to observe diffuse granular red fluorescence (AO) or the presence of vacuoles, secondary lysosomes, and residual bodies in the cytoplasm (TEM). These aspects, indicative of autophagic events in the cytoplasm of polyploid/aneuploid cells, appeared to be correlated with changes in the nuclear morphology (c), presence of chromatin aggregates (a), and fragments/micronuclei of heterogeneous size (b, d). In the image in d, the arrows indicate chromatin bridges that appear to connect the fragments to the remnant of the main nucleus. Scale bars in a and b: 5 mm; in c: 1 mm; in d: 2 µm

Back to article page

Cell Division

ISSN: 1747-1028

Contact us