Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity

Table 1 Mononuclearity, multinuclearity and main nuclear anomalies

	Small			Intermediate				Large
	4c	6c	8c	10c	12c	16c	24c	64c	110c	140c
Mononuclearity
Untreated	96	63	39	48	41	13	22	ND	ND	ND
PEG	88	67	26	33	29	7	16	0	0	0
VBL	70	ND	18	51	35	9	13	ND	ND	ND
PEG-VBL	73	ND	24	52	48	21	25	ND	0	ND
Multinuclearity
Untreated	4	37	61	52	59	87	78	ND	ND	ND
PEG	12	33	74	67	71	93	84	100	100	100
VBL	30	ND	82	49	65	91	87	ND	ND	ND
PEG-VBL	27	ND	76	48	52	79	75	ND	ND	ND
Small Micronuclei
Untreated	0	65	39	26	30	14	8	ND	ND	ND
PEG	0	63	36	22	27	15	7	0	0	0
VBL	0	ND	0	0	ND	0	ND	ND	ND	ND
PEG-VBL	0	ND	0	0	0	0	0	0	ND	ND
Nuclear budding and fragmentation
Untreated	0	0	0	2	4	9	12	ND	ND	ND
PEG	0	0	0	2	6	13	16	0	0	0
VBL	0	0	0	3	10	17	ND	ND	ND	ND
PEG-VBL	0	0	0	3	10	19	21	ND	ND	ND
Internuclear bridges
Untreated	0	2	0	4	8	7	6	ND	ND	ND
PEG	0	1	1	4	4	6	8	0	0	0
VBL	0	0	0	0	0	0	0	ND	ND	ND
PEG-VBL	0	0	0	0	0	0	0	ND	ND	ND

Example of percentage distribution of mononuclearity, multinuclearity and main nuclear anomalies in polyploid/aneuploid NIHs cells. The individual percentage values were calculated within the subpopulations with different DNA content. The nuclear features examined could occur simultaneously in the same cell. Changes of nuclear conditions were evaluated in both controls (PEG-untreated cells) and after PEG treatment. Vinblastine incubation was carried out in both PEG-untreated cells (VBL) and in previously PEG-treated cultures (PEG-VBL). To establish the percentage of various nuclear features, at least 200 cells for each DNA content value (c) were evaluated. ND: the value was Not Determined due to the absence of cells with the respective DNA content

ISSN: 1747-1028