Fig. 4

Expression of CXCR4 by IHC (n = 10). The surface of CXCR4 immunohistochemistry positive cells is brown (× 200, scale bar = 25 μm). a–e IHC of CXCR4 in the M group, M + U60s group, M + UTMD 60 s group, M + UTMD 90 s group, M + UTMD 180 s group. The number of CXCR4-positive cells was relatively smaller in the M group (a) and the M + U60s group (b), P > 0.05. The percentage of cells expressing surface CXCR4 in the M + UTMB60s group (c) higher than the M + U60s group (b) and the M group (a), P < 0.05. There was no significant difference in the number of CXCR4-positive cells between the M + UTMB60s group (c), the M + UTMB90s group (d) and the M + UTMB180s group (e), P > 0.05. f Quantification of CXCR4 expression was performed by Image-Pro Plus 6.0 software. All values are expressed as the mean ± SD. **P < 0.01; n = 10