Fig. 4

Recovery of mitosis from non-mitotic and abnormal mitotic modes of proliferation in primary cell cultures of the second recurrent HPC tumor from Case 1. The primary culture of the second recurrent tumor of Case 1 was maintained over Period 1 (Day 7–9), Period 2 (Day 10–13) and Period 3 (Day 18). A 24-h live-cell imaging was recorded for each of the three periods. A Endomitosis was the most prominent in Period 2 (2.5%) compared to Period 1 (0.4%) and Period 3 (2%). B Attempts to perform mitotic activity gradually recovered over time, from 4% at Period 1 to 11% at Period 2 then 20% at Period 3. C Mitotic level remained < 1% at Period 1 and 2, and significantly recovered to 8% at Period 3. D Frequency of DNV jumped from 0.5% at Period 1 and 2–5% at Period 3. E Cannibalism was observed at Period 1 (2%) and Period 2 (0.5%) but absent at Period 3. F Period 1 had 6% shedding activity that declined to 2% at Period 2 and 0.2% at Period 3. Mitosis are represented in blue; abnormal mitosis are in pink; failed mitosis are in hatched region and non-mitotic are in green. Statistical significance were estimate using GraphPad prism t-test to yield ***p < 0.0001; **p < 0.001; and *p < 0.01