Fig. 5

Abnormal mitotic and non-mitotic activities increased over increasing dosages of anti-mitotic drugs in cultured HeLa cells. HeLa cell cultures were treated with increasing concentrations of epirubicin (A) or cisplatin (B) as indicated on X-axis. A The mitosis level of HeLa fell in proportion to epirubicin dosage increase. The abundance of DNV in percentage under increasing dosages of epirubicin showed a reverse V-shape curve that reached peak level at 140 nM. Tailing increased along with doses and all cells were tailed at 500 nM. B Mitosis was most active at 5 μM of cisplatin then fell below 0.2-fold at higher doses compare to the 0 drug treatment. DNV showed a reversed V-shape curve that reached peak level at 5 μM. Tailing started appearing at 2 μM and gradually increased at higher dosage. Levels of mitosis, abnormal mitosis or non-mitosis in HeLa cell cultures were quantified by averaging at least two replicated experimental measurements. The dashed lines are trend-fittings to data on each panel illustrating the levels of mitosis (decreasing), abnormal mitosis (inversed V) or non-mitosis (increasing) along with increasing concentration of epirubicin (A) or cisplatin (B)