Fig. 6

Abnormal mitotic and non-mitotic activities increased over increasing dosages of anti-mitotic drugs in cultured A549 cells. A549 cell cultures were treated with increasing concentrations of epirubicin (A) or cisplatin (B) as indicated on X-axis. A Mitosis was completely compromised at all doses except 0.5 nM and 0.75 nM. DNV only occurred at 40 nM or below with the highest percentage appeared at 30–40 nM of epirubicin. Shedding appeared at the 30 nM or above and remained at a relatively low level until epirubicin dosage reached 500 nM. B Mitosis gradually fell correlated with increased dosage of cisplatin. Relative abundance of DNV showed a reversed V-shape curve and reached the highest percentage at 3.3–6.6 μM. Levels of mitosis, abnormal mitosis or non-mitosis in HeLa cell cultures were quantified by averaging at least two replicated experimental measurements. The dashed lines are trend-fittings to data on each panel illustrating the levels of mitosis (decreasing), abnormal mitosis (inversed V) or non-mitosis (increasing) along with increasing concentration of epirubicin (A) or cisplatin (B)