Fig. 1

hPTH(1–34) treatment enhanced the motility of fibroblasts and HaCaT cells. A and B Cell viability was measured by CCK8 kit after fibroblasts and HaCaT cells were subjected to the graded concentrations of hPTH(1–34) (0–1 μM) for 24 h and 72 h. C and D Representative images of wound scratching assays on fibroblasts and HaCaT cells. Images were captured at 0 h and 24 h after incubating with / without hPTH(1–34). E and F Representative images of Transwell assays on fibroblasts and HaCaT cells after incubating with / without hPTH(1–34) for 48 h. G and H Quantification of the migrated areas in hPTH(1–34)-treated fibroblasts (G) and HaCaT cells (H) after subjected to hPTH(1–34) or PBS for 24 h. I and J Quantification of the migrated fibroblasts (I) and HaCaT cells (J) after subjected to hPTH(1–34) or PBS for 48 h. The data represent mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 vs Control. Scale bar: 50 μm in (C–F). Ctrl Control