Fig. 2

hPTH(1–34) treatment induced the Rac1-related cytoskeleton re-construction and lamellipodia formation. A–D The expression of Rac1 in fibroblasts (A) and HaCaT cells (C) were evaluated by western blot analysis after treated with or without hPTH(1–34). The intensity of each band was measured and normalized to GAPDH then calculated as the ratio of the controls (B and D). E–H Cell scattering assay on fibroblasts and HaCaT cells were carried out using rhodamine-phalloidin staining after incubating with different concentrations of hPTH(1–34) for 12 h (E and G). The ratio of lamellipodia areas versus total cell areas in fibroblasts (F) and HaCaT cells (H) were measured and quantified. I Immunofluorescent staining of Rac1 and F-actin were performed on fibroblasts which were subjected to different treatments for 12 h. Green, Rac1; Red, F-actin; Blue, DAPI. The data represent mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 vs Control. Scale bar: 25 μm in (E), 10 μm in (G), 50 μm in (I). Ctrl Control