Fig. 4

Injection of hPTH(1–34) accelerated skin wound healing in rat models. A and B Full-thickness skin wounds (diameter, 15 mm) were generated on the back of rats. hPTH(1–34) or saline was locally injected around the wounds, followed by covering the wounds with sterilized dressing. Wound images were captured at day 0, 3, 7, 10, and 14 (A), and wound area at each timepoint were measured and calculated (B). C Quantitation of the wound healing time in the hPTH(1–34) and saline groups. D Immunohistochemical staining of K14 was carried out on wound samples harvested at day 7 post-surgery. E Immunofluorescence staining of Vimentin were performed on wound samples at day 7 post-surgery. F Masson’s trichrome staining was carried out on wound samples harvested at day 7 post-surgery. G Immunohistochemical staining of Rac1 was carried out on wound samples. H Measurement of the wound widths in two groups after immunohistochemical staining of K14. I Measurement of the number of Vimentin positive cells after immunofluorescence staining of Vimentin. J Measurement of the collagen intensity in two groups after Masson’s trichrome staining was carried out. K Measurement of the intensity of Rac1 expression in two groups after immunohistochemical staining of Rac1 was carried out. The data represent mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 vs saline group. Scale bar: 3 mm in (A); 1 mm in (D and F), 50 μm in (E). K14 cytokeratin 14