Fig. 2

Reduction of KCTD9 mitigates the inhibitory effect of CSE on malignant behavior in LUAD cells. a The correlation between patient prognosis’ and PON1, TGFB1, STX2, ST8SIA2, and KCTD9 in lung cancer in the Kaplan–Meier Plotter database. b The protein expression of KCTD9 in LUAD in the UALCAN database. c The mRNA expression of KCTD9 in LUAD in the UALCAN database. d Effect of CSE on KCTD9 mRNA expression in LUAD cells by RT-qPCR. e Effect of CSE on KCTD9 protein expression in LUAD cells by western blot analysis. LUAD cells were infected with sh-NC or sh-KCTD9 and treated with CSE. f Effect of sh-NC or sh-KCTD9 on KCTD9 mRNA expression in LUAD cells by RT-qPCR. g LUAD cell viability was examined using CCK-8. h LUAD cell growth was measured using a colony formation assay. i LUAD cell proliferation measured using EdU assay. j LUAD cell migration measured using wound healing assay. k LUAD cell invasion measured using Transwell invasion assay. l Detection of apoptosis in LUAD cells by flow cytometry. Data are presented as the mean ± SD of results from at least three independent experiments performed in triplicates, two-way ANOVA with Tukey’s posttest, *p < 0.05